Cooney C L, Wang D I, Mateles R I
Appl Environ Microbiol. 1976 Jan;31(1):91-8. doi: 10.1128/aem.31.1.91-98.1976.
The behavior of Enterobacter aerogenes during growth in chemostats limited by single and double nutrient restrictions was examined. On the assumption that different essential nutrients act to limit growth in different ways, we selected pairs of nutrients likely to affect different aspects of metabolism. Results show that macromolecular cell composition can be controlled by using more than one nutrient restriction. The polysaccharide content of the cells is readily manipulated by the ratio of carbon to nitrogen in the inlet nutrients. Also, at low dilution rates, ratios of protein to ribonucleic acid are dependent on the ratio of phosphate to nitrogen in the input nutrients. An examination of both acetic acid and metabolite production (as measured by ultraviolet absorbance of culture filtrates) showed that accumulation of these products was dependent on both dilution rate and type of nutrient limitation(s). These results were examined in terms of the problems of translation of batch to continuous culture processes and the use of selected nutrient limitations to control noncellular product formation.
研究了产气肠杆菌在单营养限制和双营养限制的恒化器中生长时的行为。假设不同的必需营养物质以不同方式限制生长，我们选择了可能影响代谢不同方面的营养物质对。结果表明，通过使用多种营养限制可以控制细胞的大分子组成。细胞的多糖含量很容易通过进料营养物质中碳与氮的比例来控制。此外，在低稀释率下，蛋白质与核糖核酸的比例取决于进料营养物质中磷酸盐与氮的比例。对乙酸和代谢产物生成（通过培养滤液的紫外吸光度测量）的研究表明，这些产物的积累取决于稀释率和营养限制类型。从分批培养过程向连续培养过程转化的问题以及利用选定的营养限制来控制非细胞产物形成的角度对这些结果进行了研究。
