Kawauchi S, Tsuji T, Kaku T, Kamura T, Nakano H, Tsuneyoshi M
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Am J Surg Pathol. 1998 Jan;22(1):83-92. doi: 10.1097/00000478-199801000-00011.
Sclerosing stromal tumor (SST) is a rare ovarian neoplasm occurring predominantly in young women and is histologically characterized by cellular heterogeneity, prominent vasculature, and a pseudolobular appearance composed of cellular and hypocellular areas. In the current study, three cases of SST were ultrastructurally examined and the tumors were found to be composed of several kinds of cells, i.e., luteinized thecalike cells, spindle-shaped fibroblastic cells, and primitive mesenchymal cells. These findings thus supported the ovarian stromal origin of SST. Twelve cases of SST also were analyzed immunohistochemically and demonstrated an expression of vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) in the luteinized thecalike cells and its receptor, fms-like tyrosine kinase 1 (flt-1), in capillary to medium-sized blood vessels. Reverse transcription-polymerase chain reaction (RT-PCR) also showed an expression of VPF/VEGF messenger RNA in SSTs. Accordingly, the characteristic vasculature and edema of SSTs were considered to be associated with the expression of VPF/VEGF. In addition, a fluorescence in situ hybridization (FISH) analysis also showed cells with three copy number of chromosome 12 in 13-21% of all examined SST cells, which suggested the presence of chromosome 12 trisomy in SSTs as well as in other ovarian stromal tumors.
硬化性间质瘤（SST）是一种罕见的卵巢肿瘤，主要发生于年轻女性，其组织学特征为细胞异质性、显著的血管系统以及由细胞丰富区和细胞稀少区组成的假小叶外观。在本研究中，对3例SST进行了超微结构检查，发现肿瘤由几种细胞组成，即黄素化的类卵泡膜细胞、梭形成纤维细胞和原始间充质细胞。这些发现支持了SST起源于卵巢间质。对12例SST还进行了免疫组织化学分析，结果显示黄素化的类卵泡膜细胞表达血管通透因子/血管内皮生长因子（VPF/VEGF），而在毛细血管至中等大小血管中表达其受体——fms样酪氨酸激酶1（flt-1）。逆转录-聚合酶链反应（RT-PCR）也显示SST中存在VPF/VEGF信使核糖核酸表达。因此，SST的特征性血管系统和水肿被认为与VPF/VEGF的表达有关。此外，荧光原位杂交（FISH）分析还显示，在所有检查的SST细胞中，13%至21%的细胞具有三条12号染色体拷贝数，这表明SST以及其他卵巢间质肿瘤中存在12号染色体三体。
