Hanif R, Qiao L, Shiff S J, Rigas B
Department of Medicine, Cornell University Medical College, New York, New York, USA.
J Lab Clin Med. 1997 Dec;130(6):576-84. doi: 10.1016/s0022-2143(97)90107-4.
Curcumin, the active ingredient of the rhizome of the plant turmeric (Curcuma longa Linn), a commonly used spice, prevents cancer in animal tumor models. Its mechanism of action is unknown; curcumin may act by inhibiting arachidonic acid metabolism. To explore the mechanism of curcumin's chemopreventive effect, we studied its role in proliferation and apoptosis in the HT-29 and HCT-15 human colon cancer cell lines. Curcumin dose-dependently reduced the proliferation rate of both cell lines, causing a 96% decrease by 48 hours. No apoptosis was detected. The antiproliferative effect was preceded by accumulation of the cells in the G2/M phase of cell cycle. The effect of curcumin was similar in both cell lines, which, however, differ in their ability to produce prostaglandins. We conclude that curcumin inhibits colon cancer cell proliferation in vitro mainly by accumulating cells in the G2/M phase and that this effect is independent of its ability to inhibit prostaglandin synthesis. The role of curcumin's antiproliferative effect in human colon cancer remains to be established.
姜黄素是常用香料姜黄（Curcuma longa Linn）根茎的活性成分，在动物肿瘤模型中可预防癌症。其作用机制尚不清楚；姜黄素可能通过抑制花生四烯酸代谢发挥作用。为探究姜黄素化学预防作用的机制，我们研究了其在HT - 29和HCT - 15人结肠癌细胞系增殖和凋亡中的作用。姜黄素剂量依赖性地降低了两种细胞系的增殖率，到48小时时导致96%的下降。未检测到凋亡。在细胞周期的G2/M期细胞积累之前出现了抗增殖作用。姜黄素在两种细胞系中的作用相似，不过这两种细胞系产生前列腺素的能力有所不同。我们得出结论，姜黄素在体外主要通过使细胞在G2/M期积累来抑制结肠癌细胞增殖，且这种作用与其抑制前列腺素合成的能力无关。姜黄素抗增殖作用在人类结肠癌中的作用仍有待确定。
