Connor K M, Porter J F, Quirie M M, Donachie W
Moredun Research Institute, Edinburgh, UK.
J Clin Microbiol. 1996 Mar;34(3):638-40. doi: 10.1128/jcm.34.3.638-640.1996.
Bordetella parapertussis, previously thought to be an obligate human respiratory tract pathogen, has been isolated from sheep. Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal swabbing proved futile with existing selective media because of extensive overgrowth with Mucor spp. and other nasal commensals. Moredun Bordetella Medium (MBM), which contains cycloheximide and spectinomycin at final concentrations of 0.5 mg/ml and 100 mu g/ml, respectively, was developed as an improved selective medium to isolate B. parapertussis from the nasal cavities of conventionally reared sheep. The selective ability of MBM was evaluated with 200 nasal swabs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples. MBM facilitated the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successfully excluding overgrowth with other contaminants, proved superior to other test formulations evaluated and to existing conventional media.
副百日咳博德特氏菌，以前被认为是专性人类呼吸道病原体，现已从绵羊中分离出来。由于毛霉属和其他鼻腔共生菌过度生长，用现有的选择性培养基通过鼻拭子评估传统饲养绵羊中副百日咳博德特氏菌的流行率的尝试被证明是徒劳的。莫雷登博德特氏菌培养基（MBM），其最终浓度分别含有0.5毫克/毫升的放线菌酮和100微克/毫升的壮观霉素，被开发为一种改良的选择性培养基，用于从传统饲养绵羊的鼻腔中分离副百日咳博德特氏菌。用来自传统饲养绵羊的200份鼻拭子评估了MBM的选择能力，从31.5%的样本中分离出了副百日咳博德特氏菌。MBM有助于从绵羊鼻拭子中简单有效地分离出副百日咳博德特氏菌，并且在成功排除其他污染物的过度生长方面，证明优于评估的其他测试配方和现有的传统培养基。
