来自玫瑰孢链霉菌的达托霉素基因簇的分子克隆与物理图谱分析
Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus.
作者信息
Mchenney M A, Hosted T J, Dehoff B S, Rosteck P R, Baltz R H
机构信息
Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
出版信息
J Bacteriol. 1998 Jan;180(1):143-51. doi: 10.1128/JB.180.1.143-151.1998.
Abstract
The daptomycin biosynthetic gene cluster of Streptomyces roseosporus was analyzed by Tn5099 mutagenesis, molecular cloning, partial DNA sequencing, and insertional mutagenesis with cloned segments of DNA. The daptomycin biosynthetic gene cluster spans at least 50 kb and is located about 400 to 500 kb from one end of the approximately 7,100-kb linear chromosome. We identified two peptide synthetase coding regions interrupted by a 10- to 20-kb region that may encode other functions in lipopeptide biosynthesis.
摘要
通过Tn5099诱变、分子克隆、部分DNA测序以及用克隆的DNA片段进行插入诱变，对玫瑰孢链霉菌的达托霉素生物合成基因簇进行了分析。达托霉素生物合成基因簇跨度至少50 kb，位于大约7100 kb线性染色体一端约400至500 kb处。我们鉴定出两个肽合成酶编码区，中间被一个10至20 kb的区域隔开，该区域可能在脂肽生物合成中编码其他功能。
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