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来自玫瑰孢链霉菌的达托霉素基因簇的分子克隆与物理图谱分析

Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus.

作者信息

Mchenney M A, Hosted T J, Dehoff B S, Rosteck P R, Baltz R H

机构信息

Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.

出版信息

J Bacteriol. 1998 Jan;180(1):143-51. doi: 10.1128/JB.180.1.143-151.1998.

DOI:10.1128/JB.180.1.143-151.1998
PMID:9422604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106860/
Abstract

The daptomycin biosynthetic gene cluster of Streptomyces roseosporus was analyzed by Tn5099 mutagenesis, molecular cloning, partial DNA sequencing, and insertional mutagenesis with cloned segments of DNA. The daptomycin biosynthetic gene cluster spans at least 50 kb and is located about 400 to 500 kb from one end of the approximately 7,100-kb linear chromosome. We identified two peptide synthetase coding regions interrupted by a 10- to 20-kb region that may encode other functions in lipopeptide biosynthesis.

摘要

通过Tn5099诱变、分子克隆、部分DNA测序以及用克隆的DNA片段进行插入诱变，对玫瑰孢链霉菌的达托霉素生物合成基因簇进行了分析。达托霉素生物合成基因簇跨度至少50 kb，位于大约7100 kb线性染色体一端约400至500 kb处。我们鉴定出两个肽合成酶编码区，中间被一个10至20 kb的区域隔开，该区域可能在脂肽生物合成中编码其他功能。

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本文引用的文献

1
Amino acid activation and polymerization at modular multienzymes in nonribosomal peptide biosynthesis.非核糖体肽生物合成中模块化多酶体系中的氨基酸激活和聚合。
Amino Acids. 1996 Sep;10(3):201-27. doi: 10.1007/BF00807324.
2
Lipoteichoic acid as a target for antimicrobial action.脂磷壁酸作为抗菌作用的靶点。
Microb Drug Resist. 1996 Spring;2(1):85-9. doi: 10.1089/mdr.1996.2.85.
3
Biosynthesis of acylpeptidolactones of the daptomycin type. A comparative analysis of peptide synthetases forming A21978C and A54145.达托霉素型酰基肽内酯的生物合成。形成A21978C和A54145的肽合成酶的比较分析。
Eur J Biochem. 1996 Dec 15;242(3):665-73. doi: 10.1111/j.1432-1033.1996.0665r.x.
4
Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus.利用rpsL在玫瑰孢链霉菌中进行显性选择和基因置换。
J Bacteriol. 1997 Jan;179(1):180-6. doi: 10.1128/jb.179.1.180-186.1997.
5
Biochemical characterization of peptidyl carrier protein (PCP), the thiolation domain of multifunctional peptide synthetases.多功能肽合成酶的硫醇化结构域——肽基载体蛋白（PCP）的生化特性
Chem Biol. 1996 Nov;3(11):913-21. doi: 10.1016/s1074-5521(96)90180-5.
6
A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome.一套有序的黏粒以及天蓝色链霉菌A3(2) 8兆碱基染色体的详细遗传和物理图谱。
Mol Microbiol. 1996 Jul;21(1):77-96. doi: 10.1046/j.1365-2958.1996.6191336.x.
7
Gene transfer and transposition mutagenesis in Streptomyces roseosporus: mapping of insertions that influence daptomycin or pigment production.玫瑰孢链霉菌中的基因转移和转座诱变：影响达托霉素或色素产生的插入位点定位
Microbiology (Reading). 1996 Sep;142 ( Pt 9):2363-73. doi: 10.1099/00221287-142-9-2363.
8
The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA.栖土链霉菌的不稳定区域包括位于线性染色体DNA末端两侧的210 kb末端反向重复序列。
Mol Microbiol. 1996 Jan;19(2):261-71. doi: 10.1046/j.1365-2958.1996.366894.x.
9
Engineered biosynthesis of peptide antibiotics.肽类抗生素的工程化生物合成。
Biochem Pharmacol. 1996 Jul 26;52(2):177-86. doi: 10.1016/0006-2952(96)00111-6.
10
The multiple carrier model of nonribosomal peptide biosynthesis at modular multienzymatic templates.模块化多酶模板上非核糖体肽生物合成的多载体模型。
J Biol Chem. 1996 Jun 28;271(26):15428-35. doi: 10.1074/jbc.271.26.15428.