具有胃泌酸抑制活性的人尿糖蛋白的化学和物理性质。

Chemical and physical properties of the human urinary glycoprotein with gastric antisecretory activity.

作者信息

Lugaro G, Pasta P, Casellato M M, Mazzola G, Carrea G

出版信息

Biochem J. 1976 Mar 1;153(3):641-6. doi: 10.1042/bj1530641.

DOI:10.1042/bj1530641

PMID:942377

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172633/

Abstract

The chemical and physical properties of the high-molecular-weight glycoprotein (SO20, w = 8S; Ve=Vo on Sephadex G-200) with gastric antisecretory activity extracted from the urine of pregnant women were studied. Gel filtration in the presence of sodium dodecyl sulphate and sodium dodecyl sulphate/polyacrylamide-disc-gel electrophoresis indicated subunit mol.wts. of 16 000 +/- 1500 and 13 000 +/- 1000 respectively. Reaggregation of the subunits and partial recovery of the biological activity were observed on removal of the detergent. The partial C-terminal sequence was found to be Phe-Tyr-Leu-Val-OH, whereas glycine appears to be the N-terminal amino acid. The carbohydrate composition was examined; all galactosamine was found to be O-glycosidically linked to the polypeptide chain.

摘要

对从孕妇尿液中提取的具有胃抗分泌活性的高分子量糖蛋白（SO20，w = 8S；在Sephadex G - 200上Ve = Vo）的化学和物理性质进行了研究。在十二烷基硫酸钠存在下的凝胶过滤以及十二烷基硫酸钠/聚丙烯酰胺圆盘凝胶电泳表明亚基分子量分别为16000±1500和13000±1000。去除去污剂后观察到亚基的重新聚集和生物活性的部分恢复。发现部分C末端序列为Phe - Tyr - Leu - Val - OH，而甘氨酸似乎是N末端氨基酸。对碳水化合物组成进行了检测；发现所有半乳糖胺均通过O - 糖苷键与多肽链相连。