Anasiewicz A, Maciejewski R, Juśkiewicz W, Lakowska H, Madej B, Szkodziak P
Katedry i Zakładu Anatomii Prawidłowej Człowieka Akademii Medycznej w Lublinie.
Wiad Lek. 1997;50 Suppl 1 Pt 2:96-100.
The aim of this work is to study changes in activities of lysosomal enzymes of lungs in Acute Respiratory Distress Syndrome as complication in the course of experimental acute pancreatitis. The study was carried out on 100 male rats, Wistar strain 240-480 g weight. The healthy animals were used as control to find out to norms of activity of lysosomal enzymes of lungs (Cathepsin B, D, L, Acid phosphatase, Lipase, Sulfatase, B-D-galactosidase, N-Acetyl-B-glucosaminidase). The laparotomy was performed on 90 rats and sodium taurocholate (60 animals-OP group) and 0.9% NaCl (30 animals-OK group) was injected pancreaticoduodenal duct. The rats from OP and OK groups were divided into 5 subgroups. After 2, 6, 12, 24 and 48 hours after the operation, animals were sacrificed and the lungs were dissected, weighed, frozen. After thawing the lungs in 0.9% NaCl, 1 g of lungs was sampled at ice melting point. The obtained homogenate centrifuged, supernate was poured out and centrifuged again at a speed of 14,000 rot per min. The activity of enzymes was determined in using supernate preparations by Sigma. Quantitative determination were carried out using Lowrey's method. Differences in values of enzyme activity were statistically significant, between particular groups of animals OK and OP. The highest increase in the activity of enzymes was found between 12 to 24 hours after inducing the disease, what correlated with the increase in volume of lungs. The monitoring of activity of lysosomal enzymes is a sensitive indicator to evaluate the increase of ARDS in the course of experimental acute pancreatitis.
本研究旨在探讨急性呼吸窘迫综合征（ARDS）作为实验性急性胰腺炎并发症时肺组织溶酶体酶活性的变化。实验选用100只体重240 - 480g的雄性Wistar大鼠。以健康动物作为对照，测定肺组织溶酶体酶（组织蛋白酶B、D、L、酸性磷酸酶、脂肪酶、硫酸酯酶、β - 半乳糖苷酶、N - 乙酰 - β - 葡萄糖苷酶）的活性正常范围。对90只大鼠进行剖腹手术，通过胰十二指肠导管分别注射牛磺胆酸钠（60只动物为OP组）和0.9%氯化钠溶液（30只动物为OK组）。将OP组和OK组的大鼠各分为5个亚组。术后2、6、12、24和48小时处死动物，取出肺组织，称重后冷冻保存。将肺组织在0.9%氯化钠溶液中解冻后，于冰融点取1g肺组织样本。将所得匀浆离心，弃去上清液，再以14000转/分钟的速度离心。采用Sigma公司的方法，利用上清液制剂测定酶活性。酶活性的定量测定采用Lowrey法。OK组和OP组动物之间酶活性值的差异具有统计学意义。在疾病诱发后12至24小时酶活性升高最为显著，这与肺体积的增加相关。监测溶酶体酶活性是评估实验性急性胰腺炎过程中ARDS病情加重的敏感指标。
