Wereszczyńska U, Długosz J, Gabryelewicz A, Andrzejewska A
Pathol Res Pract. 1986 Oct;181(5):576-85. doi: 10.1016/S0344-0338(86)80152-2.
The pulmonary complications are severe sequeles of acute pancreatitis. The pathogenesis of these complications is unsolved. The purpose of this work was to evaluate the status of lung lysosomes and phospholipase A activity in acute experimental pancreatitis (AEP) and the effect of heparin as a potentially protective agent. Taurocholate-induced AEP in rats lasting 24 and 48 hours was treated with heparin intraperitoneally (2 mg/kg every 8 hours). The total activity of cathepsins and B-glucuronidase in lysosomal enriched subfraction increased markedly during 48 hours of AEP in untreated animals, but the relative free activity was maximal after 24 hours. Free activity of cathepsins and acid phosphatase in supernatant was maximal after 24 hours. The phospholipase A activity was maximally elevated (more than twofold) after 48 hours. Heparin prevented the increase of activity of B-glucuronidase, depressed the relative free activity of all investigated lysosomal hydrolases and inhibited the phospholipase A activity in the lung homogenate. Our results indicate the significance of labilization of lung lysosomes and increment of phospholipase A activity in the lungs in the damage of this organ during AEP in the rats, and suggest the beneficial effect of heparin on these factors.
肺部并发症是急性胰腺炎的严重后遗症。这些并发症的发病机制尚未解决。本研究的目的是评估急性实验性胰腺炎（AEP）中肺溶酶体和磷脂酶A活性的状态，以及肝素作为一种潜在保护剂的作用。用牛磺胆酸盐诱导大鼠发生持续24和48小时的AEP，并腹腔注射肝素（每8小时2mg/kg）进行治疗。在未经治疗的动物中，AEP 48小时期间，溶酶体富集亚组分中组织蛋白酶和β-葡萄糖醛酸酶的总活性显著增加，但相对游离活性在24小时后达到最大值。上清液中组织蛋白酶和酸性磷酸酶的游离活性在24小时后达到最大值。磷脂酶A活性在48小时后最大程度升高（超过两倍）。肝素可阻止β-葡萄糖醛酸酶活性的增加，降低所有研究的溶酶体水解酶的相对游离活性，并抑制肺匀浆中的磷脂酶A活性。我们的结果表明，在大鼠AEP期间，肺溶酶体不稳定和肺中磷脂酶A活性增加在该器官损伤中具有重要意义，并提示肝素对这些因素具有有益作用。
