Onodera H, Oshima Y, Henriksen P, Yasumoto T
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Toxicon. 1997 Nov;35(11):1645-8. doi: 10.1016/s0041-0101(97)00038-x.
Cyanobacterial blooms were implicated in bird kills at lakes in Denmark in July 1993 and June-July 1994. These blooms were dominated by Anabaena lemmermannii and were shown to contain a neurotoxin with anticholinesterase activity. In this study, the toxin was isolated by mouse lethality guided column chromatographies from the field sample collected at Lake Knud sø in 1993. Various spectroscopic data indicated that the toxin was anatoxin-a(s), an irreversible anticholinesterase, first reported in Anabaena flos-aquae. Chemical detection of the same toxin in cultured A. lemmermannii also confirmed this species as the cause of the deaths of the wild birds.
1993年7月以及1994年6月至7月期间，丹麦湖泊中发生的鸟类死亡事件被认为与蓝藻水华有关。这些水华以纤细鱼腥藻为主，并且已证明含有具有抗胆碱酯酶活性的神经毒素。在本研究中，通过小鼠致死率引导的柱色谱法从1993年在克努德湖采集的野外样本中分离出了该毒素。各种光谱数据表明，该毒素是类毒素-a(s)，一种不可逆的抗胆碱酯酶，首次在水华鱼腥藻中报道。在培养的纤细鱼腥藻中对同一毒素进行化学检测，也证实了该物种是野生鸟类死亡的原因。
