尿素与醇类结合对核糖核酸酶热诱导可逆变性的影响。

The effects from combining urea and an alcohol on the heat-induced reversible denaturation of ribonuclease.

作者信息

Gerlsma S Y, Stuur E R

出版信息

Int J Pept Protein Res. 1976;8(1):3-12. doi: 10.1111/j.1399-3011.1976.tb02474.x.

DOI:10.1111/j.1399-3011.1976.tb02474.x

Abstract

The effects of a combination of an alcohol and urea on the transition temperature of bovine ribonuclease were investigated. The combined effects on the transition temperature of ribonuclease of a polyvalent alcohol and urea are about equal to the algebraic sum of the effects of each individual additive. The effects of a monovalent alcohol and urea are not cummulative, especially not at low temperatures (30 degrees C). The presence of urea decreases the hydrophobic effect of a monovalent alcohol, strongly at low temperatures, to a lesser degree at high temperatures (60 degrees C). Consequently, urea hinders the interhydrophobic interactions by affecting the water molecules.

摘要

研究了乙醇和尿素的组合对牛核糖核酸酶转变温度的影响。多元醇和尿素对核糖核酸酶转变温度的联合作用约等于各单一添加剂作用的代数和。一元醇和尿素的作用不具有累积性，尤其是在低温（30摄氏度）时。尿素的存在会降低一元醇的疏水作用，在低温时作用强烈，在高温（60摄氏度）时作用程度较小。因此，尿素通过影响水分子来阻碍疏水相互作用。