Legoedec J, Gasque P, Jeanne J F, Scotte M, Fontaine M
Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (nø. 23), INSERM U-78, BP 73, Bois-Guillaume, France.
Mol Immunol. 1997 Jul;34(10):735-41. doi: 10.1016/s0161-5890(97)00093-x.
Human myoblasts express immunological properties in vitro and we have previously reported that they produce Complement (C) components of the alternative pathway. Myoblasts activate the classical pathway but are fully protected against C attack by the expression of major C regulators. In order to fully understand the relationship between myoblasts and C, we here report the biosynthesis of C components of the classical pathway by skeletal muscle cells. Human myoblasts in vitro produced C1q, C1r, C1s, C2 and C4 constitutively and all syntheses were upregulated after stimulation with IFN-gamma. We suggest that human myoblasts may constitute a local source of C and therefore C could be implicated in inflammatory or physiopathological processes developed in skeletal muscle.
人成肌细胞在体外表现出免疫特性，我们之前曾报道它们可产生替代途径的补体（C）成分。成肌细胞可激活经典途径，但通过主要补体调节因子的表达可完全抵御补体攻击。为了全面了解成肌细胞与补体之间的关系，我们在此报告骨骼肌细胞对经典途径补体成分的生物合成。体外培养的人成肌细胞可组成性地产生C1q、C1r、C1s、C2和C4，并且在用γ干扰素刺激后所有合成均上调。我们认为人成肌细胞可能构成补体的局部来源，因此补体可能参与骨骼肌发生的炎症或病理生理过程。
