Lee C, Sarna S K
Department of Surgery, Medical College of Wisconsin, Milwaukee, USA.
Neurogastroenterol Motil. 1997 Dec;9(4):221-9. doi: 10.1046/j.1365-2982.1997.d01-58.x.
Central regulation of gastric emptying of a solid nutrient meal and spatial and temporal parameters of gastro-pyloro-duodenal contractions by corticotropin-releasing factor (CRF) were investigated in conscious dogs. Intracerebroventricular (i.c.v.) infusion of CRF at 0.033 nmol kg-1 min-1 for 15 min in a volume of 0.2 mL significantly delayed the total gastric emptying time of the meal. I.c.v infusion of CRF also increased the mean frequency of proximal duodenal contractions and decreased the percentage of distally propagating contractions in the whole duodenum. The remaining parameters of pyloric and duodenal contractions were not affected. A prior i.c.v. infusion of I-helical CRF9-41 (0.166 nmol kg-1 min-1 for 15 min in a volume of 0.2 mL) blocked the central effects of CRF on gastric emptying time and the duodenal contractions. Central infusion of CRF had no significant effect on the lag phase of gastric emptying. Bilateral truncal vagotomy significantly delayed the gastric emptying time of the solid nutrient meal. However, after vagotomy, i.c.v. infusion of CRF had no effect on gastric emptying time or the spatial and temporal parameters of gastro-pyloro-duodenal contractions. In conclusion, CRF, the mediator of stress response, delays the total gastric emptying time of solid nutrient meals. The delay in gastric emptying may not be due to a change in the spatial and temporal parameters of gastric or pyloric contractions, but mainly due to changes in the parameters of duodenal contractions. The central effects of CRF on gastric emptying and duodenal contractions may be mediated by the vagus nerves.
在清醒犬中研究了促肾上腺皮质激素释放因子（CRF）对固体营养性食物胃排空的中枢调节作用以及胃-幽门-十二指肠收缩的时空参数。以0.033 nmol·kg⁻¹·min⁻¹的剂量在0.2 mL体积内脑室内（i.c.v.）输注CRF 15分钟，显著延迟了食物的总胃排空时间。i.c.v.输注CRF还增加了十二指肠近端收缩的平均频率，并降低了整个十二指肠中向远端传播的收缩百分比。幽门和十二指肠收缩的其余参数未受影响。预先i.c.v.输注I-螺旋CRF9-41（0.166 nmol·kg⁻¹·min⁻¹，在0.2 mL体积内输注15分钟）可阻断CRF对胃排空时间和十二指肠收缩的中枢作用。中枢输注CRF对胃排空的延迟期无显著影响。双侧迷走神经切断术显著延迟了固体营养性食物的胃排空时间。然而，迷走神经切断术后，i.c.v.输注CRF对胃排空时间或胃-幽门-十二指肠收缩的时空参数无影响。总之，应激反应的介质CRF延迟了固体营养性食物的总胃排空时间。胃排空延迟可能不是由于胃或幽门收缩的时空参数变化，而是主要由于十二指肠收缩参数的变化。CRF对胃排空和十二指肠收缩的中枢作用可能由迷走神经介导。
