Hunt D E, Kelling A L, Hunt J K
Proc Soc Exp Biol Med. 1976 Feb;151(2):293-6. doi: 10.3181/00379727-151-39195.
A spectrophotometric assay was developed for measuring the uptake of the antibiotic actinobolin by hydroxylapatite (HAP) or powdered human enamel. The assay is sufficiently sensitive to detect less than 2.0 mug actinobolin/ml of: 0.01 M sodium phosphate buffer at pH 5.5, 7.0, or 8.0; deionized water; deionized water containing 1% salivary supernatant; or each of the above indicated solvent systems containing 1-5 parts per million sodium fluoride. The utility of the assay system has been demonstrated by date which show that approximately 5-7 mug of actinobolin are bound per 10 mg of HAP or powdered enamel.
已开发出一种分光光度测定法，用于测量抗生素放线杀菌素被羟基磷灰石（HAP）或人牙釉质粉末的摄取量。该测定法灵敏度足够高，能够检测到在pH值为5.5、7.0或8.0的0.01M磷酸钠缓冲液、去离子水、含1%唾液上清液的去离子水或上述每种含百万分之一至百万分之五氟化钠的溶剂系统中，每毫升低于2.0微克的放线杀菌素。该测定系统的实用性已通过数据得到证明，这些数据表明每10毫克HAP或牙釉质粉末结合约5 - 7微克的放线杀菌素。
