Richards D A
J Chromatogr. 1979 Jul 13;175(2):293-9. doi: 10.1016/s0021-9673(00)89435-2.
Electrochemical detection of tryptophan metabolites following separation on a reversed-phase high-performance liquid chromatography column was compared with other means of detection. Of 29 compounds studied, 26 could be detected at a sensitivity comparable to that of fluorescence derivatisation procedures. Response was linear over a wide range of concentrations and selectivity was shown to be superior to ultraviolet detection when analysing urine. Additionally, it was possible to control selectivity so that only those tryptophan metabolites from the tryptophan hydroxylase pathway were detected. This is of particular value in the study of distrubances of serotonin metabolism and is unique to this type of detector.
将反相高效液相色谱柱分离后的色氨酸代谢物的电化学检测与其他检测方法进行了比较。在所研究的29种化合物中，26种能够以与荧光衍生化方法相当的灵敏度被检测到。响应在很宽的浓度范围内呈线性，并且在分析尿液时，选择性优于紫外检测。此外，还可以控制选择性，使得仅检测来自色氨酸羟化酶途径的那些色氨酸代谢物。这在血清素代谢紊乱的研究中具有特殊价值，并且是这种类型的检测器所独有的。
