Xu H, Lohr J, Greiner M
Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
J Immunol Methods. 1997 Oct 13;208(1):61-4. doi: 10.1016/s0022-1759(97)00128-2.
Two hundred and sixty field serum samples were tested for Newcastle disease (ND) antibodies using a commercial enzyme-linked immunosorbent assay (ELISA) and the haemagglutination inhibition test (HI). The HI test was regarded as the reference method. Reciprocal titres of 16 and above were considered positive. In this study the two-graph receiver operating characteristic (TG-ROC) analysis was used as a tool for selecting cut-off points. Sensitivity, specificity, efficiency and Youden's index were used as indices of test accuracy. The positive and negative predictive values of the ELISA results were analysed for various prevalence rates.
使用商业酶联免疫吸附测定（ELISA）和血凝抑制试验（HI）对260份野外血清样本进行新城疫（ND）抗体检测。HI试验被视为参考方法。倒数滴度为16及以上被视为阳性。在本研究中，双图受试者工作特征（TG-ROC）分析被用作选择临界值的工具。敏感性、特异性、效率和尤登指数被用作检测准确性的指标。针对不同患病率分析了ELISA结果的阳性和阴性预测值。
