斑马鱼促甲状腺激素胚胎因子的两种亚型（tefα和tefβ）的克隆与特性分析

Cloning and characterization of two isoforms of the zebrafish thyrotroph embryonic factor (tef alpha and tefbeta).

作者信息

Xu X, Liu L, Wong K C, Ge R

机构信息

School of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.

出版信息

Biochim Biophys Acta. 1998 Jan 7;1395(1):13-20. doi: 10.1016/s0167-4781(97)00150-4.

DOI:10.1016/s0167-4781(97)00150-4

PMID:9434145

Abstract

We have cloned and sequenced two cDNAs representing two isoforms of the zebrafish thyrotroph embryonic factor (TEF) gene products (tef alpha and beta), a member of the PAR subfamily of bZIP transcription factors. The two isoforms encode two potential proteins of 300 and 293 amino acids respectively. Sequence comparison analysis indicate that the zebrafish tefs show high homology to PAR family of transcription factors of other species in the PAR domain, DNA binding domain and leucine zipper domain. Expression analysis by Northern blot and RT-PCR indicates that tef alpha and tefbeta are expressed in adult fish and throughout the zebrafish embryonic development. In addition, the two tefs are expressed in some adult tissues, but not others.

摘要

我们克隆并测序了两个cDNA，它们分别代表斑马鱼促甲状腺激素胚胎因子（TEF）基因产物的两种亚型（tefα和β），TEF是bZIP转录因子PAR亚家族的成员。这两种亚型分别编码两个潜在的蛋白质，长度分别为300和293个氨基酸。序列比较分析表明，斑马鱼的tef在PAR结构域、DNA结合结构域和亮氨酸拉链结构域与其他物种的PAR转录因子家族具有高度同源性。通过Northern印迹和RT-PCR进行的表达分析表明，tefα和tefβ在成年鱼以及斑马鱼胚胎发育全过程中均有表达。此外，这两种tef在一些成年组织中有表达，但在其他组织中不表达。