Reifferscheid G, Heil J, Oda Y, Zahn R K
Arbeitskreis Molekulare Mechanismen umweltbedingter Gentoxizität (AMMUG), Johannes Gutenberg-Universität, Mainz, F.R.G.
Mutat Res. 1991 Dec;253(3):215-22. doi: 10.1016/0165-1161(91)90134-t.
The umu-microtest is a miniaturized automated short-term test version proposed for screening of umuC-dependent mutagenic potentials of chemicals relevant to environmental pollution, river water and industrial waste water. The test is based on the SOS/umu-test and has been modified in order to allow extensive testing of environmental samples. Genetically engineered Salmonella typhimurium (TA1535/pSK1002) are incubated on a microplate rotor in a sloping position for 2 h with the test samples, followed by addition of fresh culture medium to reach a 10-fold dilution of the incubation medium. 2 h later, the activity of the beta-galactosidase, which reflects umuC induction, is determined colorimetrically. The incubation of the bacteria in the presence of the test compounds as well as the assessment of beta-galactosidase activity takes place in 96-well microplates, thus enabling simultaneous screening of large numbers of samples. Data of the genotoxic potentials are available within 8 h. Computer-controlled automation is possible by using a laboratory workstation.
umu微测试是一种小型自动化短期测试版本，旨在筛选与环境污染、河水和工业废水相关的化学物质的umuC依赖性诱变潜力。该测试基于SOS/umu测试，并进行了改进，以便对环境样品进行广泛测试。将基因工程鼠伤寒沙门氏菌（TA1535/pSK1002）在倾斜位置的微孔板转子上与测试样品孵育2小时，然后加入新鲜培养基，使孵育培养基稀释10倍。2小时后，通过比色法测定反映umuC诱导的β-半乳糖苷酶的活性。细菌在测试化合物存在下的孵育以及β-半乳糖苷酶活性的评估均在96孔微孔板中进行，从而能够同时筛选大量样品。8小时内即可获得遗传毒性潜力的数据。使用实验室工作站可以实现计算机控制的自动化。
