Przybojewska B
Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland.
Mutat Res. 1997 Nov 27;394(1-3):53-7. doi: 10.1016/s1383-5718(97)00124-1.
The alkaline (pH > 13) single cell gel electrophoresis (SCGE) assay or "comet" assay was used to evaluate for DNA damage induced in bone marrow cells of B6C3F1 mice by four aniline derivatives 2,4-dimethylaniline (2,4-xylidine). 2,4,6-trimethylaniline (mesidine), 2-chloro-4-methylaniline and 4-chloro-N-methylaniline. The study revealed that two of the four compounds studied, i.e. 2,4-dimethylaniline and 2,4,6-trimethylaniline, increased the extent of DNA migration in bone marrow cells of mice. Two others. 2-chloro-4-methylaniline and 4-chloro-N-methylaniline, had no effect on the DNA of the cells in test conditions. The results of this study, in combination with those of other researchers. leads to the conclusion that 2,4-dimethylaniline and 2,4,6-trimethylaniline are genotoxic and potentially carcinogenic.
采用碱性（pH>13）单细胞凝胶电泳（SCGE）试验，即“彗星”试验，来评估四种苯胺衍生物2,4-二甲基苯胺（2,4-二甲苯胺）、2,4,6-三甲基苯胺（联甲苯胺）、2-氯-4-甲基苯胺和4-氯-N-甲基苯胺对B6C3F1小鼠骨髓细胞造成的DNA损伤。研究表明，所研究的四种化合物中的两种，即2,4-二甲基苯胺和2,4,6-三甲基苯胺，增加了小鼠骨髓细胞中DNA的迁移程度。另外两种，2-氯-4-甲基苯胺和4-氯-N-甲基苯胺，在试验条件下对细胞DNA没有影响。这项研究的结果与其他研究人员的结果相结合，得出结论：2,4-二甲基苯胺和2,4,6-三甲基苯胺具有基因毒性且有潜在致癌性。
