Rice D W, Rafferty J B, Artymiuk P J, Lloyd R G
Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Curr Opin Struct Biol. 1997 Dec;7(6):798-803. doi: 10.1016/s0959-440x(97)80149-2.
The recent structure determination of RuvA has provided the first insights into the structural basis for its interaction with Holliday junction DNA. Multiple copies of a helix-hairpin-helix motif which line the four grooves between the monomers in the tetrameric structure are thought to be involved in the interaction of the protein with its DNA target. This suggests that the four arms of the junction are held by RuvA in a fourfold symmetric arrangement and has fuelled ideas on the way in which components of the Ruv complex combine to catalyse the process of homologous recombination.
最近对RuvA的结构测定首次揭示了其与霍利迪连接体DNA相互作用的结构基础。在四聚体结构中，排列在单体之间四条沟中的多个螺旋-发夹-螺旋基序拷贝被认为参与了该蛋白质与其DNA靶点的相互作用。这表明连接体的四条臂被RuvA以四重对称排列固定住，这引发了人们对Ruv复合体各组分如何结合以催化同源重组过程的思考。
