Crandall A D, Montville T J
Department of Food Science, Cook College, Rutgers, State University of New Jersey, New Brunswick 08901-8520, USA.
Appl Environ Microbiol. 1998 Jan;64(1):231-7. doi: 10.1128/AEM.64.1.231-237.1998.
Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype involving alterations in both the cytoplasmic membrane and the cell wall and a requirement for divalent cations. In addition to a lower ratio of C15 to C17 fatty acids than in the wild-type strain (A. S. Mazzotta and T.J. Montville, J. Appl. Microbiol. 82: 32-38, 1997), this nisin-resistant (Nisr) strain contained significantly more zwitterionic phosphatidylethanolamine and less anionic phosphatidylglycerol and cardiolipin. The extraction of cardiolipin was enhanced by a penicillin-lysozyme step to disrupt the cell wall. This study is the first to quantify the phosphatidylethanolamine component of the L. monocytogenes cytoplasmic membrane. While these cytoplasmic membrane changes were induced by nisin, the Nisr strain also showed altered sensitivities to cell wall-acting compounds, even when grown in the absence of nisin, suggesting a constitutive alteration in the strain's cell wall. A model which integrates the roles of the cell membrane, cell wall, and divalent cations is presented. Finally, nisin resistance in L. monocytogenes ATCC 700302 conferred cross-resistance to the class IIa bacteriocin pediocin PA-1 and the class IV leuconocin S.
单核细胞增生李斯特菌ATCC 700302中的乳链菌肽抗性是一种复杂的表型，涉及细胞质膜和细胞壁的改变以及对二价阳离子的需求。除了与野生型菌株相比C15与C17脂肪酸的比例较低外（A. S. 马佐塔和T. J. 蒙特维尔，《应用微生物学杂志》82: 32 - 38, 1997），这种耐乳链菌肽（Nisr）菌株含有显著更多的两性离子磷脂酰乙醇胺和更少的阴离子磷脂酰甘油及心磷脂。通过青霉素 - 溶菌酶步骤破坏细胞壁可增强心磷脂的提取。本研究首次对单核细胞增生李斯特菌细胞质膜中的磷脂酰乙醇胺成分进行了定量。虽然这些细胞质膜变化是由乳链菌肽诱导的，但Nisr菌株对作用于细胞壁的化合物的敏感性也发生了改变，即使在无乳链菌肽的情况下生长也是如此，这表明该菌株的细胞壁存在组成性改变。本文提出了一个整合细胞膜、细胞壁和二价阳离子作用的模型。最后，单核细胞增生李斯特菌ATCC 700302中的乳链菌肽抗性赋予了对IIa类细菌素片球菌素PA - 1和IV类亮菌素S的交叉抗性。
