Moore L A, Broihier H T, Van Doren M, Lunsford L B, Lehmann R
Skirball Institute, New York University Medical Center, NY, NY 10016, USA.
Development. 1998 Feb;125(4):667-78. doi: 10.1242/dev.125.4.667.
Gonadogenesis in the Drosophila embryo is a complex process involving numerous cellular migratory steps and cell-cell interactions. The mechanisms guiding germ cells to move through, recognize and adhere to specific cell types are poorly understood. In order to identify genes that are required for these processes, we have conducted an extensive mutagenesis of the third chromosome and screened for mutations disrupting germ cell migration at any point in embryonic development. Phenotypic analysis of these mutants demonstrates that germ cell migration can be broken down into discrete developmental steps, with each step requiring a specific set of genes. Many of these genes are involved in the development of gonadal mesoderm, the tissue that associates with germ cells to form the embryonic gonad. Moreover, mutations that we isolated affecting embryonic patterning as well as germ cell migration suggest that the origin of gonadal mesoderm lies within the eve domain of the developing mesoderm.
果蝇胚胎中的性腺发生是一个复杂的过程，涉及众多细胞迁移步骤和细胞间相互作用。引导生殖细胞移动、识别并黏附特定细胞类型的机制目前还知之甚少。为了鉴定这些过程所需的基因，我们对第三条染色体进行了广泛的诱变，并筛选在胚胎发育任何阶段破坏生殖细胞迁移的突变。对这些突变体的表型分析表明，生殖细胞迁移可分解为离散的发育步骤，每个步骤都需要一组特定的基因。其中许多基因参与性腺中胚层的发育，该组织与生殖细胞结合形成胚胎性腺。此外，我们分离出的影响胚胎模式形成以及生殖细胞迁移的突变表明，性腺中胚层的起源位于发育中胚层的eve区域内。
