Hierholzer J C, Tannock G A
J Med Virol. 1979;4(4):279-90. doi: 10.1002/jmv.1890040406.
Three type-specific hemagglutinins of adenovirus (AV) 4 were purified, tested for various activities, and used as immunogens in rabbits. The components were purified by anion exchange and exclusion chromatographies to purification factors of 33, 20, and 37, respectively, and were identified as fiber (polypeptide, mol wt 70,000; incomplete hemagglutinin), penton (mol wt greater than 400,000; incomplete hemagglutinin; possession of cytotoxic activity), and dodecon (mol wt greater than 400,000; complete hemagglutinin; appearance by electron microscopy). Rabbit antisera to these hemagglutinating components possessed type-specific hemagglutination-inhibition and serum neutralization antibodies and were thoroughly evaluated for potency and specificty in immunodiffusion (ID), immunoelectrophoresis, and counterelectrophoresis (CE) tests. The anti-fiber and anti-dodecon sera were highly type-specific in ID and CE tests with a battery of commonly isolated AV serotypes; the anti-penton serum was similarly specific after it was absorbed with AV 2 hexons. These sera were thus proved to be suitable reagents for use in both the routine diagnostic ID test and the more rapid CE test.
纯化了腺病毒（AV）4的三种型特异性血凝素，对其进行了各种活性测试，并用作兔的免疫原。通过阴离子交换色谱法和排阻色谱法分别将这些成分纯化至纯化因子为33、20和37，并鉴定为纤维（分子量70,000的多肽；不完全血凝素）、五邻体（分子量大于400,000；不完全血凝素；具有细胞毒性活性）和十二聚体（分子量大于400,000；完全血凝素；通过电子显微镜观察到）。针对这些血凝成分的兔抗血清具有型特异性血凝抑制和血清中和抗体，并在免疫扩散（ID）、免疫电泳和对流电泳（CE）试验中对效价和特异性进行了全面评估。在对一系列常见分离的AV血清型进行ID和CE试验时，抗纤维和抗十二聚体血清具有高度型特异性；抗五邻体血清在用AV 2六邻体吸收后同样具有特异性。因此，这些血清被证明是适用于常规诊断ID试验和更快速的CE试验的试剂。
