Bramucci M, Miano A, Gobbetti A, Zerani M, Quassinti L, Maccari E, Murri O, Amici D
Department of Molecular, Cellular and Animal Biology, University of Camerino, MC, Italy.
Am J Physiol. 1997 Dec;273(6):R2089-96. doi: 10.1152/ajpregu.1997.273.6.R2089.
Our aim was to study the role of angiotensin-converting enzyme (ACE) and angiotensin II (ANG II) on ovarian steroidogenesis and prostaglandin production of amphibian. Hormonal effects of ACE, ACE inhibitors, synthetic bullfrog angiotensin I (ANG I), and [Val5]ANG II were compared on frog ovaries of postreproductive and prereproductive periods. Very high ACE activity was found in ovary of water frog (Rana esculenta) compared with other frog tissues, and this activity was inhibited by the typical ACE inhibitors, captopril and lisinopril. Frog ovary tissue in postreproductive and prereproductive periods was incubated in vitro in the presence of ACE (2.5 mU/ml), captopril (0.1 mM), lisinopril (0.1 mM), [Val5]ANG II (1 microM), and synthetic bullfrog ANG I (1 microM). Production of 17 beta-estradiol, progesterone, androgens, and prostaglandins E2 and F2 alpha was determined. The data showed a modulation of 17 beta-estradiol, progesterone, and prostaglandin E2 production by ovary ACE; on the other hand, [Val5]ANG II modulated the production of progesterone and prostaglandin F2 alpha, whereas androgen production was not influenced. The present in vitro studies suggest the existence of two pathways independently regulated by ACE and ANG II modulating ovarian steroidogenesis and prostaglandin production.
我们的目的是研究血管紧张素转换酶（ACE）和血管紧张素II（ANG II）在两栖动物卵巢类固醇生成和前列腺素产生中的作用。比较了ACE、ACE抑制剂、合成牛蛙血管紧张素I（ANG I）和[Val5]ANG II对繁殖后期和繁殖前期青蛙卵巢的激素作用。与其他青蛙组织相比，在水蛙（食用蛙）卵巢中发现了非常高的ACE活性，并且这种活性受到典型ACE抑制剂卡托普利和赖诺普利的抑制。将繁殖后期和繁殖前期的青蛙卵巢组织在体外与ACE（2.5 mU/ml）、卡托普利（0.1 mM）、赖诺普利（0.1 mM）、[Val5]ANG II（1 microM）和合成牛蛙ANG I（1 microM）一起孵育。测定了17β-雌二醇、孕酮、雄激素以及前列腺素E2和F2α的产生。数据显示卵巢ACE对17β-雌二醇、孕酮和前列腺素E2的产生有调节作用；另一方面，[Val5]ANG II调节孕酮和前列腺素F2α的产生，而雄激素的产生不受影响。目前的体外研究表明存在两条由ACE和ANG II独立调节的途径，它们调节卵巢类固醇生成和前列腺素产生。
