Baskurt O K, Temiz A, Meiselman H J
Department of Physiology and Biophysics, USC Medical School, Los Angeles, CA 90033, USA.
Free Radic Biol Med. 1998 Jan 1;24(1):102-10. doi: 10.1016/s0891-5849(97)00169-x.
The human red blood cell (RBC) is known to be susceptible to oxidant damage, with both structural and functional properties altered consequent to oxidant attack. Such oxidant-related alterations may lead to changes of RBC rheologic behavior (i.e., deformability, aggregability). Two different models of oxidant stress were used in this study to generate superoxide anions either internal or external to the RBC. Our results indicate that generation of superoxide within the RBC by phenazine methosulfate decreases RBC deformability without effects on cell aggregation. Conversely, superoxide generated externally by the xanthine oxidase-hypoxanthine system primarily affects RBC aggregability: the shear rate necessary to disaggregate RBC was markedly increased while the extent of aggregation decreased slightly. Increased disaggregation shear rate (i.e., greater aggregate strength) as a result of superoxide radical damage may adversely affect the dynamics of blood flow in low-shear portions of the circulation, and may also play a role in the no-reflow phenomena encountered after ischemia-reperfusion.
已知人类红细胞（RBC）易受氧化损伤，氧化攻击会导致其结构和功能特性发生改变。这种与氧化相关的改变可能会导致红细胞流变行为（即变形性、聚集性）的变化。本研究使用两种不同的氧化应激模型在红细胞内部或外部产生超氧阴离子。我们的结果表明，硫酸吩嗪在红细胞内产生超氧阴离子会降低红细胞变形性，但对细胞聚集没有影响。相反，黄嘌呤氧化酶-次黄嘌呤系统在外部产生的超氧阴离子主要影响红细胞聚集性：使红细胞解聚所需的剪切速率显著增加，而聚集程度略有下降。超氧自由基损伤导致的解聚剪切速率增加（即更大的聚集强度）可能会对循环中低剪切部分的血流动力学产生不利影响，也可能在缺血再灌注后出现的无复流现象中起作用。
