纤连蛋白III型结构域中微秒时间尺度上普遍存在的构象波动。
Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain.
作者信息
Akke M, Liu J, Cavanagh J, Erickson H P, Palmer A G
机构信息
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
出版信息
Nat Struct Biol. 1998 Jan;5(1):55-9. doi: 10.1038/nsb0198-55.
A novel off-resonance rotating-frame 15N NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 microseconds in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the beta-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the beta-sheets. Flexibility of the loop containing the Arg-Gly-Asp (RGD) tripeptide may affect the accessibility of this motif in protein-protein interactions.
一种新型的非共振旋转框架15N核磁共振自旋弛豫实验被用于表征人腱生蛋白-C的第三个III型纤连蛋白结构域中相关时间在32至175微秒之间的构象波动。III型结构域β-折叠结构连续区域的构象波动可能代表集体运动，例如β-折叠的瞬时扭曲或呼吸。包含精氨酸-甘氨酸-天冬氨酸（RGD）三肽的环的灵活性可能会影响该基序在蛋白质-蛋白质相互作用中的可及性。
Zotero 插件