Nagae H, Takahashi H, Kuroki Y, Honda Y, Nagata A, Ogasawara Y, Abe S, Akino T
Immunology Laboratory, Yamasa Corporation, Chiba, Japan.
Clin Chim Acta. 1997 Oct 31;266(2):157-71. doi: 10.1016/s0009-8981(97)00124-1.
We developed an enzyme-linked immunosorbent assay (ELISA) for detection of SP-D in serum using recombinant SP-D as a standard and horseradish peroxidase conjugated F(ab')2 fragment of mouse monoclonal antibody IgG to avoid the interaction of serum factors including rheumatoid factor. The use of F(ab')2 fragment dramatically decreased the value of serum SP-D concentration in rheumatoid arthritis patients without pulmonary complication to the close level of healthy volunteer. In contrast, the patients with collagen disease having interstitial pulmonary pneumonia exhibited consistently elevated levels of serum SP-D. The use of new ELISA with recombinant SP-D and F(ab')2 fragment of anti-SP-D monoclonal antibody gives a greater advantage for the accurate detection of SP-D in sera from patients with idiopathic pulmonary fibrosis, interstitial pneumonia with collagen disease and pulmonary alveolar proteinosis without interference of rheumatoid factor.
我们开发了一种酶联免疫吸附测定法（ELISA），以重组SP-D为标准品，用辣根过氧化物酶偶联的小鼠单克隆抗体IgG的F(ab')2片段来检测血清中的SP-D，以避免包括类风湿因子在内的血清因子的相互作用。使用F(ab')2片段可显著降低无肺部并发症的类风湿关节炎患者血清SP-D浓度值，使其接近健康志愿者的水平。相比之下，患有间质性肺炎的胶原病患者血清SP-D水平持续升高。使用含有重组SP-D和抗SP-D单克隆抗体F(ab')2片段的新型ELISA，在检测特发性肺纤维化、胶原病伴间质性肺炎和肺泡蛋白沉积症患者血清中的SP-D时，不受类风湿因子干扰，具有更大优势。
