Chmielnicki Z, Was M, Kmieciak-Kołada K, Huzarska M, Spiewak Z, Pawłowski J, Kamiński M, Dyaczyńska-Herman A, Herman Z S
Department of Anesthesiology and Intensive Care, Silesian University School of Medicine, Katowice, Poland.
Pol J Pharmacol. 1997 Mar-Jun;49(2-3):97-106.
The objective of this study was to analyse the effects of isoflurane anesthesia (lasting for 15 or 60 min) and isoflurane anesthesia termination (after 1 or 24 h) on met-enkephalin (MENK) and leu-enkephalin (LENK) levels in discrete brain areas and spinal cord segments in rabbits. Moreover histochemical analysis of activities of succinate dehydrogenase, magnesium-dependent adenosine triphosphatase (Mg++ATP-ase) and acid phosphatase in the striatum and hypothalamus were carried out to evaluate the effects of isoflurane anesthesia on energetic, transport and catabolic processes. Throughout anesthesia (15 and 60 min) and after its termination (1 h) the LENK contents were increased in hypothalamus, hippocampus, mesencephalon and lumbar segment of spinal cord. Moreover, during isoflurane anesthesia and after its termination (1 h) MENK and LENK levels decreased in cervical segment and MENK content dropped in thoracic segment of spinal cord. Histochemical data indicated, that isoflurane enhanced energetic processes as well as exchange processes in neurocytes, glial cells, capillary walls and ependymal cells of the third ventricle. Measurements of acid phosphatase activity provided evidence of no signs of toxicity of isoflurane in the examined areas. The changes in enkephalin levels observed during the isoflurane anesthesia and after its termination depended on the type of examined neuropeptides, as well as on parts of the brain and spinal cord studied. The changes observed after isoflurane administration in enkephalinergic system are discussed with regard to our earlier experiments with halothane and enflurane.
本研究的目的是分析异氟烷麻醉（持续15或60分钟）及异氟烷麻醉终止（1或24小时后）对兔离散脑区和脊髓节段中甲硫氨酸脑啡肽（MENK）和亮氨酸脑啡肽（LENK）水平的影响。此外，还对纹状体和下丘脑的琥珀酸脱氢酶、镁依赖性三磷酸腺苷酶（Mg++ATP酶）及酸性磷酸酶活性进行了组织化学分析，以评估异氟烷麻醉对能量、转运和分解代谢过程的影响。在整个麻醉期间（15和60分钟）及其终止后（1小时），下丘脑、海马、中脑和脊髓腰段的LENK含量增加。此外，在异氟烷麻醉期间及其终止后（1小时），脊髓颈段的MENK和LENK水平降低，脊髓胸段的MENK含量下降。组织化学数据表明，异氟烷增强了第三脑室神经细胞、神经胶质细胞、毛细血管壁和室管膜细胞中的能量过程以及交换过程。酸性磷酸酶活性的测定提供了证据表明在所检查区域没有异氟烷毒性迹象。在异氟烷麻醉期间及其终止后观察到的脑啡肽水平变化取决于所检查的神经肽类型，以及所研究的脑和脊髓部位。结合我们早期使用氟烷和恩氟烷的实验，讨论了异氟烷给药后在脑啡肽能系统中观察到的变化。
