Su T, Mulla M S
Department of Entomology, University of California, Riverside 92521-0314, USA.
J Med Entomol. 1997 Nov;34(6):729-34. doi: 10.1093/jmedent/34.6.729.
Autogenous and anautogenous strains were selected from the egg rafts of Culex tarsalis Coquillett collected from Coachella Valley, California. In autogenous (21-25 generations) and anautogenous (0-4 generations) strains used for hemolymph free amino acid analysis, the autogenous oviposition rates were 73.6-83.6% and 2.7-3.9%, the proportions of females undergoing autogenous ovarian development 120 h postemergence were 75.6 and 8.9%, respectively. To study the presence and quantities of free amino acids and explore their relationship to autogenous ovarian development, the 6 legs of females from both autogenous and anautogenous strains were removed at the coxo-femoral joints, and hemolymph was collected by centrifuging the bodies of 50-100 females. Proteins in the hemolymph were precipitated with 10% sulphosalicylic acid. On days 1, 4, 7, and 10 postemergence, a total of 17 free amino acids was found in females from the autogenous strain, and a total of 16 was found in females from the anautogenous strain in which asparagine (Asn) was absent. Comparisons of free amino acid concentrations between these 2 strains indicated that there were significant differences in total free amino acids and 13 individual free amino acids except Glu, Gln, Pro, and Met on day 1, total free amino acids and 14 individual free amino acids except Gln, Pro, and on day 4, and total free amino acids and 12 individual free amino acids except Asp, Glu, Gln, Pro, and Met on day 7 postemergence. There were no significant differences in the concentrations of individual and total free amino acids on day 10 postemergence. Female age affected free amino acid content in the autogenous strain, where total free amino acids and 14 individual free amino acid content in the autogenous strain, where total free amino acids and 14 individual free amino acids increased from day 1, peaked on day 4, declined on day 7, and declined further on day 10 postemergence. In the anautogenous strain, only total free amino acids and Ser, Gly, Ala, and Leu exhibited age-dependent changes in concentration, and the magnitude of changes in concentration was less than that in autogenous strain. Conclusively, autogenous ovarian development was the major reason for the changes in hemolymph free amino acid concentration postemergence. The current and previous findings indicate that the free amino acids, Arg, Gly, Ile, Leu, Lys, Phe, Ser, Thr, and Val putatively may be essential for vitellogenesis in mosquitoes.
从加利福尼亚州科切拉谷采集的致倦库蚊卵块中挑选出自生性和非生性品系。在用于血淋巴游离氨基酸分析的生性（21 - 25代）和非生性（0 - 4代）品系中，生性品系的产卵率为73.6 - 83.6%，非生性品系为2.7 - 3.9%；羽化后120小时进行生性卵巢发育的雌蚊比例分别为75.6%和8.9%。为研究游离氨基酸的存在情况和数量，并探索它们与生性卵巢发育的关系，将生性和非生性品系雌蚊的6条腿在髋 - 股关节处切除，通过离心50 - 100只雌蚊的身体来收集血淋巴。血淋巴中的蛋白质用10%的磺基水杨酸沉淀。在羽化后的第1、4、7和10天，生性品系雌蚊中共检测到17种游离氨基酸，非生性品系雌蚊中总共检测到16种游离氨基酸（其中不含天冬酰胺）。这两个品系游离氨基酸浓度的比较表明，羽化后第1天，除了Glu、Gln、Pro和Met外，总游离氨基酸和13种单个游离氨基酸存在显著差异；第4天，除了Gln和Pro外，总游离氨基酸和14种单个游离氨基酸存在显著差异；第7天，除了Asp、Glu、Gln、Pro和Met外，总游离氨基酸和12种单个游离氨基酸存在显著差异。羽化后第10天，单个和总游离氨基酸浓度没有显著差异。雌蚊年龄影响生性品系中的游离氨基酸含量，生性品系中的总游离氨基酸和14种单个游离氨基酸含量从第1天开始增加，在第4天达到峰值，第7天下降，第10天进一步下降。在非生性品系中，只有总游离氨基酸以及Ser、Gly、Ala和Leu的浓度呈现出随年龄的变化，且浓度变化幅度小于生性品系。总之，生性卵巢发育是羽化后血淋巴游离氨基酸浓度变化的主要原因。当前和之前的研究结果表明，游离氨基酸Arg、Gly、Ile、Leu、Lys、Phe、Ser、Thr和Val可能对蚊子的卵黄发生至关重要。
