Nakashita H, Watanabe K, Hara O, Hidaka T, Seto H
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
J Antibiot (Tokyo). 1997 Mar;50(3):212-9.
The biosynthetic step following the phosphoenolpyruvate (PEP) phosphomutase reaction which forms a C-P bond of bialaphos was proven by the identification of phosphonopyruvate (PnPy) and phosphonoacetaldehyde (PnAA) as intermediates in the culture broth of Streptomyces hygroscopicus, a producing organism of bialaphos, and by detection of enzymatic decarboxylation of PnPy to PnAA. Purified PnPy decarboxylase turned out to require thiamine diphosphate and Mg2+ as cofactors. PnPy decarboxylase drives the unfavorable forward reaction to form PnPy catalyzed by PEP phosphomutase and is suggested to be essential to C-P compound biosynthesis.
通过鉴定膦丙酮酸（PnPy）和膦乙醛（PnAA）作为双丙氨膦产生菌吸水链霉菌培养液中的中间体，并检测PnPy向PnAA的酶促脱羧反应，证实了在形成双丙氨膦C-P键的磷酸烯醇丙酮酸（PEP）磷酸变位酶反应之后的生物合成步骤。纯化的PnPy脱羧酶结果表明需要硫胺素二磷酸和Mg2+作为辅因子。PnPy脱羧酶推动由PEP磷酸变位酶催化的形成PnPy的不利正向反应，并且被认为对于C-P化合物生物合成至关重要。
