Fanuel-Barret D, Patrice T, Foultier M T, Vonarx-Coinsmann V, Robillard N, Lajat Y
Laboratoire de Médecine, Ecole Nationale Vétérinaire de Nantes, France.
Res Exp Med (Berl). 1997;197(4):219-33. doi: 10.1007/s004330050071.
Photodynamic therapy (PDT) could be a useful adjuvant in glioblastoma treatment. The fact that epidermal growth factor (EGF) and its receptor are involved in glioblastoma growth control led us to investigate the relationships between EGF and PDT with respect to three different glioma cell lines (C6, T98 G, U87 MG) responsive to growth stimulation by EGF. Flow cytometric analysis revealed that each cell line expressed EGF receptors. PDT was then applied to the cells using haematoporphyrin derivative (HPD) as photosensitizer and argon laser irradiation. When cells were incubated for 2 h with HPD (0.1-10 micrograms/ml) and then laser-irradiated (lambda = 514 nm; energy density 25 J/cm2), all three cell lines showed photosensitivity. The median lethal dose was respectively 3, 4.5 and 2.7 micrograms/ml for C6, T98 G and U87 MG. EGF (2-50 ng/ml) had no effect on HPD- and laser-induced toxicity when added to cells before PDT, whereas toxicity decreased for all three cell lines when EGF was added after PDT. HPD (1-2 micrograms/ml, incubation times 30-180 min) also induced an increase in EGF receptor expression for the C6 line.
光动力疗法（PDT）可能是胶质母细胞瘤治疗中一种有用的辅助疗法。表皮生长因子（EGF）及其受体参与胶质母细胞瘤生长控制这一事实促使我们研究在三种对EGF生长刺激有反应的不同胶质瘤细胞系（C6、T98 G、U87 MG）中EGF与PDT之间的关系。流式细胞术分析显示每个细胞系都表达EGF受体。然后使用血卟啉衍生物（HPD）作为光敏剂并进行氩激光照射，将PDT应用于细胞。当细胞与HPD（0.1 - 10微克/毫升）孵育2小时，然后进行激光照射（波长= 514纳米；能量密度25焦/平方厘米）时，所有三个细胞系均表现出光敏性。C6、T98 G和U87 MG的半数致死剂量分别为3、4.5和2.7微克/毫升。在PDT前将EGF（2 - 50纳克/毫升）添加到细胞中时，对HPD和激光诱导的毒性没有影响，而在PDT后添加EGF时，所有三个细胞系的毒性均降低。HPD（1 - 2微克/毫升，孵育时间30 - 180分钟）也使C6细胞系的EGF受体表达增加。
