Aglas F, Rainer F, Lipp R W, Schnedl W J, Horn S, Egger G
Department of Internal Medicine, Karl-Franzens University, Graz, Austria.
Rheumatol Int. 1997;17(4):137-40. doi: 10.1007/s002960050024.
The influence of methylprednisolone on the migratory characteristics of neutrophil granulocytes was investigated in 10 patients with early rheumatoid arthritis (RA) and compared to 12 controls. The migration of neutrophils was measured with a whole-blood membrane filter assay with and without stimulation by the chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP). Total migration index (TMI), distribution characteristics (DC) and the product of TMI and DC (neutrophil migratory activity; NMA) served to characterize the migratory behaviour of neutrophils. The data demonstrated an increased polymorphonuclear leucocyte (PMN) migration in patients with early RA, indicating a bystander role of PMNs in inflammatory joint injury. Treatment with methylprednisolone reduced significantly the penetration depth (DC) of neutrophils, but did not influence the number of migrating cells (TMI). The unstimulated NMA was significantly reduced due to the marked DC reduction, whereas steroids did not influence the stimulated NMA of neutrophils. A significant reduction in PMN penetration depth was demonstrated only after a steroid therapy of at least 10 days, suggesting that a longer period of steroid therapy is necessary to provide effective inflammatory control.
在10例早期类风湿关节炎（RA）患者中研究了甲基强的松龙对中性粒细胞迁移特性的影响，并与12名对照者进行了比较。采用全血膜滤过试验，在有和没有趋化因子N-甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）刺激的情况下，测定中性粒细胞的迁移情况。总迁移指数（TMI）、分布特征（DC）以及TMI与DC的乘积（中性粒细胞迁移活性；NMA）用于表征中性粒细胞的迁移行为。数据表明，早期RA患者的多形核白细胞（PMN）迁移增加，表明PMN在炎症性关节损伤中起旁观者作用。甲基强的松龙治疗显著降低了中性粒细胞的穿透深度（DC），但不影响迁移细胞的数量（TMI）。由于DC显著降低，未受刺激的NMA显著降低，而类固醇不影响中性粒细胞受刺激后的NMA。仅在至少10天的类固醇治疗后，PMN穿透深度才出现显著降低，这表明需要更长时间的类固醇治疗才能有效控制炎症。
