Hashimoto H, Yamamura M, Nishiya K, Ota Z
Third Department of Internal Medicine, Okayama University Medical School, Japan.
Acta Med Okayama. 1994 Aug;48(4):181-7. doi: 10.18926/AMO/31089.
The accumulation of polymorphonuclear leukocytes (PMN) in synovial fluid is a common feature of rheumatoid arthritis (RA). We studied the chemotactic response of PMN obtained from the synovial fluid and from the peripheral blood of patients with RA using a modified Boyden's method, in which interleukin-8 (IL-8) or N-formyl-methionyl-leucyl-phenylalanine (FMLP) was used as a chemotactic agent. The IL-8-induced response of peripheral blood PMN from 15 patients with RA did not differ from that of 15 healthy controls. A decreased chemotactic response to IL-8 was, however, observed in PMN from the synovial fluid of 12 patients with RA compared with peripheral blood cells of the same individual. This defective chemotactic ability of PMN was inversely correlated with the number of infiltrating cells in the synovial fluid. We also obtained similar results with FMLP. These results indicate that the chemotactic ability of PMN may be reduced after migrating to the synovial fluid.
多形核白细胞（PMN）在滑液中的积聚是类风湿关节炎（RA）的一个常见特征。我们使用改良的博伊登方法，研究了从RA患者的滑液和外周血中获取的PMN的趋化反应，其中白细胞介素-8（IL-8）或N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）用作趋化剂。15例RA患者外周血PMN对IL-8诱导的反应与15例健康对照者无异。然而，与同一个体的外周血细胞相比，在12例RA患者滑液中的PMN中观察到对IL-8的趋化反应降低。PMN这种有缺陷的趋化能力与滑液中浸润细胞的数量呈负相关。我们用FMLP也得到了类似结果。这些结果表明，PMN迁移到滑液后其趋化能力可能会降低。
