加州海兔中5-羟色胺和多巴胺受体的年龄依赖性变化。

Age dependent changes in serotonin and dopamine receptors in Aplysia californica.

作者信息

Southall M D, Flinn J M, Holt R W, Chandhoke V

机构信息

Department of Biology, George Mason University, Fairfax, VA 22030-4444, USA.

出版信息

Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1997 Oct;118(2):137-41. doi: 10.1016/s0742-8413(97)00109-6.

DOI:10.1016/s0742-8413(97)00109-6

PMID:9440239

Abstract

Age related changes in dopaminergic and serotonergic receptors were examined in Aplysia californica. In this study dopamine (DA) and serotonin (5-HT) receptor levels were examined for animals belonging to 4-, 5-, 6-, 8-, 9- and 12-month age groups. Receptors analysis was performed using radio-labeled d-[3H] lysergic acid diethylamide (LSD) as the specific ligand. Specific binding for 5-HT was found to be significantly greater than that for DA in the young (4-month post-hatch) animals. The total DA and 5-HT receptor levels changed significantly with age. Dopamine levels increased from 5.34 fmol/mg of protein at 4 months to 19.11 fmol/mg at 12 months. Serotonin receptor levels increased from 7.35 fmol/mg at 4 months to 20.45 at 12 months.

摘要

对加州海兔的多巴胺能和5-羟色胺能受体的年龄相关变化进行了研究。在本研究中，检测了4个月、5个月、6个月、8个月、9个月和12个月龄组动物的多巴胺（DA）和5-羟色胺（5-HT）受体水平。使用放射性标记的d-[3H]麦角酸二乙胺（LSD）作为特异性配体进行受体分析。在幼龄（孵化后4个月）动物中，发现5-HT的特异性结合显著高于DA。总DA和5-HT受体水平随年龄显著变化。多巴胺水平从4个月时的5.34 fmol/mg蛋白质增加到12个月时的19.11 fmol/mg。5-羟色胺受体水平从4个月时的7.35 fmol/mg增加到12个月时的20.45。