硒代氨基酸和有机硒化合物的高效液相色谱法。电感耦合等离子体质谱法进行形态分析。
High-performance liquid chromatography of selenoamino acids and organo selenium compounds. Speciation by inductively coupled plasma mass spectrometry.
作者信息
Bird S M, Ge H, Uden P C, Tyson J F, Block E, Denoyer E
机构信息
Department of Chemistry, Lederle Graduate Research Tower A, University of Massachusetts, Amherst 01003-4510, USA.
出版信息
J Chromatogr A. 1997 Nov 21;789(1-2):349-59. doi: 10.1016/s0021-9673(97)00657-2.
As part of an ongoing study to identify selenium compounds with cancer chemopreventive activity, extracts of selenium-enriched samples were analyzed by HPLC-inductively coupled plasma (ICP)-MS. Ion-exchange, ion pair and derivatization methods for reversed-phase HPLC were considered and advantages and disadvantages for each compared. Anion exchange allows separation of selenite and selenate, but otherwise provides poor separation. Pre-column derivatization and reversed-phase chromatography provides separation of compounds with terminal amine functionalities, but many other species elute in the void volume. The ion pair method gave optimal separation and was compatible with standard ICP-MS operating conditions.
作为一项正在进行的旨在鉴定具有癌症化学预防活性的硒化合物的研究的一部分，采用高效液相色谱-电感耦合等离子体质谱法（HPLC-ICP-MS）对富硒样品的提取物进行了分析。考虑了反相高效液相色谱的离子交换、离子对和衍生化方法，并比较了每种方法的优缺点。阴离子交换可分离亚硒酸盐和硒酸盐，但除此之外分离效果不佳。柱前衍生化和反相色谱可分离具有末端胺官能团的化合物，但许多其他物质在死体积中洗脱。离子对方法实现了最佳分离，并且与标准ICP-MS操作条件兼容。
Zotero 插件