[寡脱氧核糖核苷硫代磷酸酯的高效液相色谱法]
[HPLC of oligodeoxyribonucleoside phosphorothioates ].
作者信息
Metelev V N, Tashlitskił V N, Agraval S
出版信息
Bioorg Khim. 1997 Sep;23(9):742-6.
PMID:9441597
Abstract
Conditions are described for the separation of synthetic oligodeoxyribonucleoside phosphorothioates by HPLC using a SCOUT WP PEI weak anion exchanger and for the separation of oligodeoxyribonucleotides with mixed phosphate and phosphorothioate internucleotide bonds on a Partisphere C18 support in ion-pair conditions. The influence of the nucleoside composition and the number of phosphorothioate bonds on the retention time was studied.
摘要
描述了使用SCOUT WP PEI弱阴离子交换剂通过高效液相色谱法分离合成寡脱氧核糖核苷硫代磷酸酯的条件，以及在离子对条件下在Partisphere C18载体上分离具有磷酸酯和硫代磷酸酯核苷酸间键的寡脱氧核苷酸的条件。研究了核苷组成和硫代磷酸酯键数量对保留时间的影响。
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