Belongia BM, Baygents JC
Department of Chemical and Environmental Engineering, The University of Arizona, Tucson, Arizona, 85721
J Colloid Interface Sci. 1997 Nov 1;195(1):19-31. doi: 10.1006/jcis.1997.5131.
Taylor-Aris dispersion in narrow-bore capillaries is used to measure the diffusion coefficient of colloidal particles in aqueous suspension. The method is shown to yield accurate results for particles up to about 0.3 &mgr;m in diameter; the measurement time for larger particles is prohibitively long and impractical. For hydrophobic particles, interactions with the capillary walls can introduce error into the interpretation of the data. The measurements also suggest that buoyancy-driven particle motion can introduce error. Consequently, a method similar to capillary hydrodynamic fractionation was developed to establish when these factors were of negligible effect. The results constitute an order-and-a-half improvement in the sensitivity of the technique, which has been recently shown to work for nanometer-sized proteins. The data suggest that, when matched with the appropriate theory, dispersion in capillaries may be a useful probe of colloidal and gravitational interaction potentials. Copyright 1997 Academic Press. Copyright 1997Academic Press
窄孔毛细管中的泰勒-阿里斯扩散用于测量水悬浮液中胶体颗粒的扩散系数。结果表明，该方法对于直径约0.3微米以下的颗粒能产生准确的结果；对于较大颗粒，测量时间长得令人望而却步且不切实际。对于疏水性颗粒，与毛细管壁的相互作用会给数据解释带来误差。测量还表明，浮力驱动的颗粒运动会引入误差。因此，开发了一种类似于毛细管流体动力学分级的方法，以确定这些因素何时影响可忽略不计。结果表明该技术的灵敏度提高了一到半级，最近已证明该技术对纳米级蛋白质有效。数据表明，当与适当的理论匹配时，毛细管中的扩散可能是胶体和引力相互作用势的有用探针。版权所有1997年学术出版社。版权所有1997年学术出版社
