用于具有生物学意义的胶体颗粒电泳迁移率的毛细管电泳测量。
Capillary Electrophoresis Measurements of Electrophoretic Mobility for Colloidal Particles of Biological Interest.
作者信息
Glynn JR, Belongia BM, Arnold RG, Ogden KL, Baygents JC
机构信息
Department of Chemical and Environmental Engineering, The University of Arizona, Tucson, Arizona 85721.
出版信息
Appl Environ Microbiol. 1998 Jul 1;64(7):2572-7. doi: 10.1128/AEM.64.7.2572-2577.1998.
The electrophoretic mobilities of three bacterial strains were investigated by capillary electrophoresis (CE) and were compared with results obtained by microelectrophoresis (ME). The CE measurements yielded bimodal electropherograms for two of the strains, thus illustrating for the first time that surface charge variations within a monoclonal population can be probed by CE. Intrapopulation variations were not detected by ME. The mobilities of three chemically distinct types of latex microspheres were also measured. Differences between the mean mobilities obtained by CE and ME were not statistically significant (P </= 0.50); the standard deviations of the CE measurements were typically 2 to 10 times smaller than those obtained by comparable ME measurements. The reproducibility of CE permitted batch-to-batch mobility variations to be probed for the bacteria (one of the strains exhibited such variations), and aggregation was evident in one of the latex suspensions. These effects were not measurable with ME.
通过毛细管电泳（CE）研究了三种细菌菌株的电泳迁移率，并与通过微电泳（ME）获得的结果进行了比较。CE测量结果显示，其中两种菌株的电泳图谱呈双峰状，从而首次表明，CE可以探测单克隆群体内的表面电荷变化。ME未检测到群体内的变化。还测量了三种化学性质不同的乳胶微球的迁移率。CE和ME获得的平均迁移率之间的差异无统计学意义（P≤0.50）；CE测量的标准差通常比通过类似ME测量获得的标准差小2至10倍。CE的可重复性使得能够探测细菌批次间的迁移率变化（其中一种菌株表现出这种变化），并且在一种乳胶悬浮液中明显存在聚集现象。ME无法测量这些效应。
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