Davey P, Barza M, Peckman C
Invest Ophthalmol Vis Sci. 1987 May;28(5):867-73.
We compared the growth patterns of gram-negative bacilli in the vitreous humor (experimental endophthalmitis in rabbits) and in a subcutaneous site of infection (croton oil pouches in rats). In untreated animals, inoculation of Klebsiella pneumoniae or Pseudomonas aeruginosa into either site was followed by a period of rapid bacterial multiplication. Thereafter, the numbers of bacteria in the vitreous humor fell spontaneously, whereas those in the subcutaneous site remained stable. During treatment with antibiotics, there was a decline in the numbers of bacteria in both sites. However, once the drug had been eliminated, the numbers of bacteria remained low in the vitreous humor but increased in the subcutaneous site. These findings suggest that during the course of infection, there was depletion of an essential bacterial nutrient or accumulation of an antibacterial substance in the vitreous humor but not in the subcutaneous site. To examine some of these possibilities, we made biochemical measurements during the course of untreated infection. In general, the biochemical changes in the two sites were similar except that the pH fell to about 6.6-6.8 in the vitreous humor but remained above 7.0 in the subcutaneous site. None of the biochemical changes that we observed seemed likely to account for the spontaneous decline in bacterial numbers in the vitreous humor. Further study is warranted to determine the cause of the antibacterial effect in the vitreous humor during the course of experimental bacterial endophthalmitis.
我们比较了革兰氏阴性杆菌在玻璃体液（兔实验性眼内炎）和皮下感染部位（大鼠巴豆油袋）的生长模式。在未治疗的动物中，将肺炎克雷伯菌或铜绿假单胞菌接种到任一部位后，细菌都会有一段快速繁殖期。此后，玻璃体液中的细菌数量会自发下降，而皮下部位的细菌数量则保持稳定。在使用抗生素治疗期间，两个部位的细菌数量都有所下降。然而，一旦药物被清除，玻璃体液中的细菌数量仍然很低，而皮下部位的细菌数量则增加了。这些发现表明，在感染过程中，玻璃体液中存在必需细菌营养物质的消耗或抗菌物质的积累，而皮下部位则没有。为了研究其中的一些可能性，我们在未治疗的感染过程中进行了生化测量。一般来说，两个部位的生化变化相似，只是玻璃体液的pH值降至约6.6 - 6.8，而皮下部位的pH值保持在7.0以上。我们观察到的生化变化似乎都不太可能解释玻璃体液中细菌数量的自发下降。有必要进行进一步研究以确定实验性细菌性眼内炎过程中玻璃体液抗菌作用的原因。
