Kövér K E, Hruby V J, Uhrín D
Department of Organic Chemistry, L. Kossuth University, Debrecen, H-4010, Hungary.
J Magn Reson. 1997 Dec;129(2):125-9. doi: 10.1006/jmre.1997.1265.
A pulsed field gradient version of the sensitivity-enhanced 2D HSQC-TOCSY experiment is proposed for measurement of long-range heteronuclear coupling constants. The coupling constants are obtained by computer-aided analysis of mixed-phase multiplets with and without the heteronuclear splitting. Generation of pure phase data is not required. Since large 1JXH and JHH couplings are used for coherence transfer, small nJXH can be measured accurately, which could be difficult to obtain from purely heteronuclear polarization transfer experiments.
提出了一种灵敏度增强的二维HSQC - TOCSY实验的脉冲场梯度版本，用于测量远程异核耦合常数。通过对具有和不具有异核分裂的混合相多重峰进行计算机辅助分析来获得耦合常数。不需要生成纯相数据。由于使用大的(^{1}J_{XH})和(J_{HH})耦合进行相干转移，因此可以准确测量小的(^{n}J_{XH})，而这从纯异核极化转移实验中可能难以获得。
