Osterlund A, Engstrand L
Department of Clinical Microbiology, University Hospital, Uppsala, Sweden.
Acta Otolaryngol. 1997 Nov;117(6):883-8. doi: 10.3109/00016489709114219.
A novel method for the in vitro culture of tissue biopsies is presented. Infected tonsillar biopsies were successfully cultured by means of the continuous flow of media without the addition of antibiotics. Histological and immunohistological examinations were carried out on sections of Streptococcus pyogenes-infected cultured tonsillar biopsies and sections from the surgically removed tonsils of asymptomatic S. pyogenes carriers. Low numbers of intracellular S. pyogenes were detected inside epithelial cells in eight out of 12 (67%) S. pyogenes-infected biopsies, and in four out of 11 (36%) tonsils of asymptomatic carriers. Our findings support the hypothesis that the sanctuary where S. pyogenes avoids antibacterial treatment is intracellular, which may provide a possible explanation of recurrent tonsillitis.
提出了一种用于组织活检体外培养的新方法。通过培养基的连续流动，在不添加抗生素的情况下成功培养了感染的扁桃体活检组织。对化脓性链球菌感染的培养扁桃体活检组织切片以及无症状化脓性链球菌携带者手术切除扁桃体的切片进行了组织学和免疫组织学检查。在12例化脓性链球菌感染的活检组织中有8例（67%）以及11例无症状携带者的扁桃体中有4例（36%），在上皮细胞内检测到少量细胞内化脓性链球菌。我们的研究结果支持这样一种假说，即化脓性链球菌逃避抗菌治疗的庇护所是细胞内，这可能为复发性扁桃体炎提供一种可能的解释。
