用于检测弓形虫感染患者血清中抗弓形虫IgG的三种免疫学检测方法的组合

Combinations of three immunological assays for detecting anti-Toxoplasma IgG in the sera of patients infected with Toxoplasma gondii.

作者信息

Liu Y S, Du W P, Xue J Q, Chen M, Ma Q Y, Zhang S H, Wang J F, Liu J, Wu Z X

机构信息

Xuzhou Medical College, Xuzhou, Jiangsu, PR China.

出版信息

Southeast Asian J Trop Med Public Health. 1997 Jun;28(2):335-8.

PMID:9444016

Abstract

Enzyme-linked immunosorbent assay (ELISA), Dot-ELISA and Dot-immunogold silver staining (Dot-IGSS) were simultaneously used to detect the specific IgG against Toxoplasma gondii in 65 patients infected with the protozoa. The positive rates were 86.51%, 92.51% and 98.64%, respectively. When ELISA and Dot-ELISA results were put together, the positive rate increased to 95.38%. When Dot-IGSS results were combined with those of ELISA or Dot-ELISA, the positive rate was raised to 100%. The difference in positive rate between ELISA and Dot-IGSS was significant (x2 = 6.93, p < 0.01), but no statistically significant differences were found between ELISA and Dot-ELISA or between Dot-ELISA and Dot-IGSS. Paired comparison of the reacting intensities of the sera in the 3 assays showed the correlations were highly significant (p < 0.001), with r = 0.608 between Dot-IGSS and Dot-ELISA, r = 0.8194 between Dot-IGSS and ELISA and r = 0.517 between Dot-ELISA and ELISA. Hence combination of different serological assays may increase their sensitivity and specificity for detecting the anti-Toxoplasma antibodies.

摘要

采用酶联免疫吸附试验（ELISA）、斑点酶联免疫吸附试验（Dot-ELISA）和斑点免疫金-银染色法（Dot-IGSS）同时检测65例弓形虫感染患者血清中抗弓形虫特异性IgG。其阳性率分别为86.51%、92.51%和98.64%。ELISA和Dot-ELISA结果合并后，阳性率增至95.38%。Dot-IGSS结果与ELISA或Dot-ELISA结果合并后，阳性率提高到100%。ELISA与Dot-IGSS阳性率差异有统计学意义（x2 = 6.93，p < 0.01），但ELISA与Dot-ELISA之间以及Dot-ELISA与Dot-IGSS之间差异无统计学意义。对3种检测方法血清反应强度进行配对比较，结果显示相关性高度显著（p < 0.001），Dot-IGSS与Dot-ELISA之间r = 0.608，Dot-IGSS与ELISA之间r = 0.8194，Dot-ELISA与ELISA之间r = 0.517。因此，联合应用不同血清学检测方法可提高抗弓形虫抗体检测的敏感性和特异性。