Paca-uccaralertkun S, Chuchottaworn C
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1997 Jun;28(2):387-90.
Isoniazid resistant mechanisms in Mycobacterium tuberculosis have been shown to involve at least two genes, kat G and inh A. Alteration in the kat G gene has been found in a great number of resistant isolates. Percentage of resistant isolates harboring alteration in this gene varied among laboratories suggesting that different mutations were presented in different geographic areas. Fourteen isoniazid resistant and five multidrug resistant isolates from the Central Chest Hospital, Thailand, were examined for the kat G gene mutations in the region between base position 17 to 299. No different pattern of mutations were found between these two groups. Among nineteen isolates, there were nine isolates which showed point mutations and five isolates with base insertions of the kat G gene. The remaining five isolates revealed gene deletion. Heteroduplex formation technique also confirmed base alterations in these nine mutants.
结核分枝杆菌对异烟肼的耐药机制已被证明至少涉及两个基因，即kat G和inh A。在大量耐药菌株中发现了kat G基因的改变。不同实验室中该基因发生改变的耐药菌株百分比有所不同，这表明不同地理区域存在不同的突变。对来自泰国中央胸部医院的14株异烟肼耐药菌株和5株多重耐药菌株进行了检测，分析其kat G基因在第17至299位碱基之间区域的突变情况。这两组菌株未发现不同的突变模式。在这19株菌株中，有9株表现出点突变，5株有kat G基因的碱基插入。其余5株显示基因缺失。异源双链形成技术也证实了这9个突变体中的碱基改变。
