Sabbatini A, Dolcher M P, Marchini B, Chimenti D, Moscato S, Pratesi F, Bombardieri S, Migliorini P
Clinical Immunology Unit, University of Pisa, Italy.
Clin Exp Rheumatol. 1997 Nov-Dec;15(6):655-8.
To study the specificity of antibodies reactive with renal antigens in mixed cryoglobulinemia.
Sera from mixed cryoglobulinemia (MC) patients were tested on human kidney extracts by immunoblot. A partially purified renal antigen was subjected to N-terminal sequencing.
Antibodies reactive with a renal antigen of 48 kD were detected in 7 out of 11 patients with MC and renal involvement. N-terminal sequencing of this antigen showed that it was identical with alpha-enolase. This result was confirmed by the reactivity of the renal antigen with a rabbit anti-serum specific for alpha-enolase.
The results indicate that antibodies specific for alpha-enolase are frequently produced by mixed cryoglobulinemia patients with renal involvement.
研究混合性冷球蛋白血症中与肾抗原反应的抗体的特异性。
通过免疫印迹法检测混合性冷球蛋白血症（MC）患者血清与人肾提取物的反应。对部分纯化的肾抗原进行N端测序。
在11例有肾受累的MC患者中，7例检测到与48 kD肾抗原反应的抗体。该抗原的N端测序表明它与α-烯醇化酶相同。肾抗原与抗α-烯醇化酶兔抗血清的反应性证实了这一结果。
结果表明，有肾受累的混合性冷球蛋白血症患者经常产生针对α-烯醇化酶的特异性抗体。
