Marinho C F, Costa-Maia J, Pinto-de-Barros J, Oliveira C R
Center for Neuroscience of Coimbra, School of Medicine, University of Coimbra, Portugal.
Eur Arch Psychiatry Clin Neurosci. 1997;247(5):275-7. doi: 10.1007/BF02900306.
A cross-sectional survey was undertaken to study the aging effect on platelet cytoplasmic membrane outer leaflet fluidity and Na+/H+ antiporter activity, and to investigate the relative influence of membrane order and age on the Na+/H+ exchanger. The study population consisted of 19 healthy subjects (age range 22-83 years, mean age 48.5 +/- 16.5 years; 12 females and 7 males). Washed platelets were used as neuronal models and as eventually contributing to brain pathology by aberrant secretion of their granule content. A significant positive correlation (r = 0.48, p < 0.05) was found between age and the membrane structural order, according to a second-degree equation. Sewall-Wright path coefficient analysis revealed that the age influence on Na+/H+ exchanger activity was unimportant (0.18), but those of cytoplasmic membrane outer leaflet fluidity and mean platelet volume were strong and positive (0.51 and 0.54, respectively). Women presented a marked standard deviation in the countertransporter results. In conclusion, decreased membrane outer leaflet fluidity and unchanged Na+/H+ antiporter activity may contribute to the process of normal brain aging.
开展了一项横断面调查，以研究衰老对血小板细胞质膜外小叶流动性和Na⁺/H⁺反向转运体活性的影响，并探讨膜有序性和年龄对Na⁺/H⁺交换体的相对影响。研究人群包括19名健康受试者（年龄范围22 - 83岁，平均年龄48.5±16.5岁；12名女性和7名男性）。洗涤后的血小板被用作神经元模型，并且可能因其颗粒内容物的异常分泌而导致脑部病变。根据二次方程，年龄与膜结构有序性之间存在显著正相关（r = 0.48，p < 0.05）。休厄尔 - 赖特路径系数分析表明，年龄对Na⁺/H⁺交换体活性的影响不显著（0.18），但细胞质膜外小叶流动性和平均血小板体积的影响强烈且为正（分别为0.51和0.54）。女性在反向转运体结果中呈现出明显的标准差。总之，膜外小叶流动性降低和Na⁺/H⁺反向转运体活性不变可能有助于正常脑衰老过程。
