Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
School of Dentistry, National Taiwan University, Taipei, Taiwan.
Sci Rep. 2021 Dec 14;11(1):23954. doi: 10.1038/s41598-021-03434-1.
To investigate the effect of Er:YAG laser treatment on lipopolysaccharide (LPS) clearance and fibroblast adhesion on titanium disks. Grade IV titanium discs (n = 216) were used and allocated to 6 groups. Group 1 was the negative control without Porphyromonas gingivalis inoculation. Discs in Groups 2-6 were incubated with P. gingivalis to form a biofilm. Group 3 received 0.12% chlorhexidine irrigation and Group 4 received titanium curettage to remove the biofilm. Group 5 was treated with Er:YAG laser irradiation and Group 6 was treated with titanium curettage plus Er:YAG laser irradiation. The contact angle and surface roughness were measured after the various treatments. The surface microstructure and residual bacteria were examined using scanning electron microscopy and confocal laser scanning microscopy, respectively. Residual LPS was examined using a limulus amoebocyte lysate assay and human gingival fibroblast adhesion was quantified using fluorescent microscopy. Curettage plus Er:YAG laser irradiation was the most effective method for removing bacteria and LPS. No significant difference in the amount of fibroblast adhesion was found between the control and Group 6. Combined use of Er:YAG laser irradiation and curettage optimizes LPS clearance and fibroblast adhesion on titanium discs.
研究 Er:YAG 激光处理对钛盘上脂多糖 (LPS) 清除和成纤维细胞黏附的影响。使用四级钛盘（n=216），并将其分为 6 组。第 1 组为未接种牙龈卟啉单胞菌的阴性对照。第 2-6 组的圆盘与牙龈卟啉单胞菌孵育以形成生物膜。第 3 组接受 0.12%洗必泰冲洗，第 4 组接受钛刮除术以去除生物膜。第 5 组用 Er:YAG 激光照射处理，第 6 组用钛刮除术加 Er:YAG 激光照射处理。在各种处理后测量接触角和表面粗糙度。使用扫描电子显微镜和共聚焦激光扫描显微镜分别检查表面微观结构和残留细菌。使用鲎变形细胞溶解物测定法检查残留 LPS，并用荧光显微镜定量测定人牙龈成纤维细胞黏附。刮除术加 Er:YAG 激光照射是去除细菌和 LPS 的最有效方法。与对照组相比，第 6 组的成纤维细胞黏附量无显著差异。Er:YAG 激光照射和刮除术的联合使用优化了钛盘上 LPS 的清除和成纤维细胞黏附。
