亚最小抑菌浓度的氨苄西林、氯霉素和呋喃妥因对体外培养的大肠杆菌黏附人尿道上皮细胞的影响。

Effects of subminimal inhibitory concentrations of ampicillin, chloramphenicol, and nitrofurantoin on the attachment of Escherichia coli to human uroepithelial cells in vitro.

作者信息

Sandberg T, Stenqvist K, Svanborg-Edén C

出版信息

Rev Infect Dis. 1979 Sep-Oct;1(5):838-44. doi: 10.1093/clinids/1.5.838.

DOI:10.1093/clinids/1.5.838

Abstract

An in vitro system with epithelial cells of the human urinary tract and Escherichia coli isolated from the urine of pateints with acute pyelonephritis was used for tests of the effect of antibacterial agents on bacterial adhesion. E. coli treated with subminimal inhibitory amounts of ampicillin attached less than untreated control bacteria. Subminimal inhibitory amounts of chloramphenicol and nitrofurantoin had no effect on adhesion of the 15 strains of E. coli tested. The combination of bacterial pretreatment with ampicillin and addition of antibodies to the adhesion test tube decreased adhesion more than did either treatment separately.

摘要

利用一种体外系统进行抗菌剂对细菌黏附作用的测试，该系统包含人泌尿道上皮细胞以及从急性肾盂肾炎患者尿液中分离出的大肠杆菌。用低于最小抑菌量的氨苄西林处理的大肠杆菌，其黏附能力低于未处理的对照细菌。低于最小抑菌量的氯霉素和呋喃妥因对所测试的15株大肠杆菌的黏附没有影响。在黏附测试管中，先用氨苄西林对细菌进行预处理并添加抗体，这种联合处理比单独使用任何一种处理方法都能更有效地降低细菌黏附。