Scaramuzzi R J, Baird D T
J Reprod Fertil. 1976 Jan;46(1):39-47. doi: 10.1530/jrf.0.0460039.
Six ewes were immunized against a prostaglandin F-2alpha-protein conjugate. Between 24 and 82 days after immunization the regular cyclic occurrence of oestrus was abolished in all six ewes. Further investigations of the immunized animals revealed that the blockade of oestrus was due to a persistence of the CL and the constantly elevated (greater than 2 ng/ml) blood levels of progesterone. Surgical enucleation of the persistent CL was promptly followed by a fall in progesterone concentrations (less than 0-5 ng/ml), normal oestrus and a subsequent return to a state of constantly elevated blood progesterone levels. These results show that neutralization of the biological activity of PGF by active immunization against PGF-2alpha results in a failure of luteal regression and provide evidence that endogenous PGF is involved in normal luteal regression in this species.
六只母羊用前列腺素F-2α-蛋白质结合物进行免疫。免疫后24至82天期间，所有六只母羊的发情周期均停止。对免疫动物的进一步研究表明，发情受阻是由于黄体持续存在以及孕酮血液水平持续升高（大于2纳克/毫升）。对持续存在的黄体进行手术摘除后，孕酮浓度迅速下降（小于0.5纳克/毫升），恢复正常发情，随后血液孕酮水平又持续升高。这些结果表明，通过对前列腺素F-2α进行主动免疫来中和其生物活性会导致黄体退化失败，并证明内源性前列腺素参与了该物种的正常黄体退化过程。
