粘质沙雷氏菌DNA促旋酶gyrA基因的克隆、核苷酸序列分析及喹诺酮耐药临床分离株gyrA基因突变特征
Cloning and nucleotide sequence of the DNA gyrase gyrA gene from Serratia marcescens and characterization of mutations in gyrA of quinolone-resistant clinical isolates.
作者信息
Kim J H, Cho E H, Kim K S, Kim H Y, Kim Y M
机构信息
Department of Pharmacology and Molecular Biology, C&C Research Laboratories, Kyunggi-do, Korea.
出版信息
Antimicrob Agents Chemother. 1998 Jan;42(1):190-3. doi: 10.1128/AAC.42.1.190.
Abstract
The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined. An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.
摘要
测定了粘质沙雷氏菌ATCC 14756的DNA促旋酶gyrA基因序列。发现一个2640个核苷酸的开放阅读框，编码一个计算分子量为97460的多肽，其序列补充了大肠杆菌gyrA温度敏感突变的序列。对来自六个喹诺酮耐药临床分离株的gyrA基因喹诺酮耐药决定区的PCR产物分析显示，所有六个突变体中均有一个氨基酸取代，即Ser-83突变为Arg或Asp-87突变为Tyr，这表明gyrA的突变改变是粘质沙雷氏菌喹诺酮耐药的常见机制。
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