Adeyemo O, Gao R J, Lan H C
Department of Pathobiology, School of Veterinary Medicine, Tuskegee University, AL 36088, USA.
Cell Mol Biol (Noisy-le-grand). 1997 Nov;43(7):1031-7.
The response of caprine macrophages to exposure to caprine arthritis-encephalitis virus (CAEV) and lipopolysaccharide (LPS) was investigated in female Nubian and Nubian crossbreed of goats. Macrophages were matured in vitro from monocytes isolated from blood of control and CAEV-infected goats and the concentrations of tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6) in culture supernatant after exposure of cells to LPS and virus were assayed. Though barely detectable in unstimulated cells, TNF-alpha and IL-6 levels showed peak values of 420 +/- 28 to 530 +/- 32 and 70 +/- 27 to 93 +/- 29, respectively, in supernatants of control goat cells [corrected], remaining high through 24 hrs. post treatment with LPS stimulation. Exposure of these control goat cells [corrected] to virus induced lower secretions (p<0.05) of the cytokines. The peak values occurred between 6 and 12 hrs. post treatment with LPS or virus. Cells prepared from virus-infected goats and treated with the mitogen or virus showed significantly (p<0.05) lower response than those from control goats. The present results suggest a dysregulation, possibly downregulation of the production of both cytokines in macrophages of goats chronically diseased by lentivirus infection.
在努比亚母山羊及其杂交品种中，研究了山羊巨噬细胞对山羊关节炎-脑炎病毒（CAEV）和脂多糖（LPS）暴露的反应。巨噬细胞在体外由从对照山羊和感染CAEV的山羊血液中分离的单核细胞成熟而来，并在细胞暴露于LPS和病毒后，测定培养上清液中肿瘤坏死因子（TNF-α）和白细胞介素-6（IL-6）的浓度。尽管在未刺激的细胞中几乎检测不到，但对照山羊细胞[校正后]的上清液中，TNF-α和IL-6水平分别显示出420±28至530±32和70±27至93±29的峰值，并在LPS刺激处理后24小时内一直保持较高水平。这些对照山羊细胞[校正后]暴露于病毒后，细胞因子的分泌较低（p<0.05）。在用LPS或病毒处理后6至12小时出现峰值。从感染病毒的山羊制备的细胞并用丝裂原或病毒处理后，其反应明显（p<0.05）低于对照山羊的细胞。目前的结果表明，在慢病毒感染的慢性患病山羊的巨噬细胞中，两种细胞因子的产生可能存在失调，可能是下调。
