Mahmoud S A, Abdel-Hafez A M, Mashhoor W A, Refaat A A
Zentralbl Bakteriol Naturwiss. 1978;133(2):115-20. doi: 10.1016/s0323-6056(78)80021-4.
Attempts were made for using industrial and agricultural by-products and wastes as carbon and nitrogen sources in fermentation medium for alpha-amylase production by Aspergillus niger NRRL-337. The original carbon source of the basal medium was replaced by one of the following materials: rice bran, wheat bran, corn bran, corn starch, cane molasses, and glucose syrup. Rice bran proved to be the best carbon source that secured the highest amylase activity. The nitrogen source of the basal medium was then replaced by different cheap materials, viz: dried yeast, corn steep liquor, gluten-30, gluten-50, and corn steep precipitate. Corn steep precipitate proved to be superior in amylase production. In consideration of these results an economical medium that secured high activity, containing the following ingredients, was suggested: 2.5% corn steep precipitate, 7.2% rice bran, 0.1% MgSO4, 0.1% KH2PO4, and 0.1% CaCO3. From this medium fungal amylase was precipitated and purified. The pure enzyme gave the highest activity at 40 degrees C and pH 4.3.
人们尝试利用工农业副产品和废弃物作为发酵培养基中的碳源和氮源，用于黑曲霉NRRL-337生产α-淀粉酶。基础培养基的原始碳源被以下材料之一取代：米糠、麦麸、玉米麸、玉米淀粉、甘蔗糖蜜和葡萄糖浆。事实证明，米糠是确保淀粉酶活性最高的最佳碳源。然后，基础培养基的氮源被不同的廉价材料取代，即：干酵母、玉米浆、面筋-30、面筋-50和玉米浆沉淀。事实证明，玉米浆沉淀在淀粉酶生产方面表现更优。考虑到这些结果，建议使用一种能确保高活性的经济培养基，其成分如下：2.5%玉米浆沉淀、7.2%米糠、0.1%硫酸镁、0.1%磷酸二氢钾和0.1%碳酸钙。从这种培养基中沉淀并纯化出真菌淀粉酶。纯酶在40摄氏度和pH值4.3时活性最高。
